A0481
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 | Immunoblotting Cell lysates of HEK-293T transfected with Mouse Axin1-FLAG expression vector, were separated on SDS-PAGE and blotted with Anti-Axin1 (C-terminal) (Cat. No. A0481). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody dilution 1:25,000 2: Antibody dilution 1:50,000 3: Antibody dilution 1:25,000 + Inhibition with Axin1 immunizing peptide (rat, 815-832) (10 μg/mL) 4: Untransfected HEK-293T cells. Antibody dilution 1:25,000 |
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A0481
Sigma
Anti-Axin1 (C-terminal) antibody produced in rabbit
1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Axin, Anti-Axis inhibition protein 1, Anti-Axis Inhibitor 1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~130 kDa |
| species reactivity | mouse, rat |
| concentration | 1.5 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1. |
| Gene Information | human ... AXIN1(8312) |
| rat ... Axin1(79257) | |
| mouse ... Axin1(12005) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
